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cd150 sirna (Bioneer Corporation)

Name: cd150 sirna
Brand: Bioneer Corporation
Rating: 90 (1 reviews)

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Bioneer Corporation cd150 sirna
Sequences of the oligonucleotide primers used for RT-PCR and RT-qPCR.

Cd150 Sirna, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd150 sirna/product/Bioneer Corporation
Average 90 stars, based on 1 article reviews

cd150 sirna - by Bioz Stars, 2026-02

90/100 stars

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1) Product Images from "CD150-dependent activation of EBV-transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines"

Article Title: CD150-dependent activation of EBV-transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines

Journal: International Journal of Molecular Medicine

doi: 10.3892/ijmm.2024.5403

Figure Legend Snippet: Sequences of the oligonucleotide primers used for RT-PCR and RT-qPCR.

Techniques Used:

Figure Legend Snippet: siRNAs used in transfection.

Techniques Used: Transfection, Negative Control

Dynamics of CD150 expression in EBVt-B cells. Resting B cells purified from human peripheral blood were immortalized with medium containing EBV particles. (A) Weekly flow cytometry analysis of EBVt-B cells stained with PE-conjugated anti-human CD150 and FITC-conjugated anti-human CD20 antibodies. The left panels show surface expression and the right panels show intracellular expression. (B) Confocal microscopy analysis showing the upregulation of CD150 expression and colocalization of CD150 with CD20 on the cell surface and in the cytoplasm of EBVt-B cells. Scale bars, 10 μ m. (C) Reverse transcription-quantitative PCR results showing CD150 mRNA levels relative to β-actin at 4 weeks post infection. * P<0.05, ** P<0.01 vs. naïve B cells. Data are presented as the mean ± SD of three replicates and were analyzed using one-way ANOVA followed by Scheffe's test. The results represent three independent experiments. EBV, Epstein-Barr virus; EBVt-B, EBV-transformed B (cells); PBMCs, peripheral blood mononuclear cells; wk, week.

Figure Legend Snippet: Dynamics of CD150 expression in EBVt-B cells. Resting B cells purified from human peripheral blood were immortalized with medium containing EBV particles. (A) Weekly flow cytometry analysis of EBVt-B cells stained with PE-conjugated anti-human CD150 and FITC-conjugated anti-human CD20 antibodies. The left panels show surface expression and the right panels show intracellular expression. (B) Confocal microscopy analysis showing the upregulation of CD150 expression and colocalization of CD150 with CD20 on the cell surface and in the cytoplasm of EBVt-B cells. Scale bars, 10 μ m. (C) Reverse transcription-quantitative PCR results showing CD150 mRNA levels relative to β-actin at 4 weeks post infection. * P<0.05, ** P<0.01 vs. naïve B cells. Data are presented as the mean ± SD of three replicates and were analyzed using one-way ANOVA followed by Scheffe's test. The results represent three independent experiments. EBV, Epstein-Barr virus; EBVt-B, EBV-transformed B (cells); PBMCs, peripheral blood mononuclear cells; wk, week.

Techniques Used: Expressing, Purification, Flow Cytometry, Staining, Confocal Microscopy, Reverse Transcription, Real-time Polymerase Chain Reaction, Infection, Virus, Transformation Assay

Effects of CD150 stimulation on inflammatory cytokine secretion. (A) Representative quantitative levels of cytokines produced by EBVt-B cells at 24 h after CD150 stimulation with antibody cross-linking, as determined using multiplex cytokine ELISA. (B) Reverse-transcription-quantitative PCR analysis of various inflammatory cytokines at 3, 6, 12 and 24 h after CD150 stimulation with antibody cross-linking in EBVt-B cells. (C) Time course of the quantification of cytokine protein production by EBVt-B cells. (D) Representative quantitative levels of cytokine production in the EBV + Burkitt lymphoma cell lines, IM-9 and Raji at 24 h after CD150 stimulation. * P<0.05, ** P<0.01 vs. mock stimulated with the control UPC-10 Ab. Data are presented as the mean ± SD of three replicates and were analyzed using one-way ANOVA followed by Scheffe's test. All results represent three independent experiments. EBVt-B, Epstein-Barr virus-transformed B (cells); GM-CSF, granulocyte-macrophage colony-stimulating factor.

Figure Legend Snippet: Effects of CD150 stimulation on inflammatory cytokine secretion. (A) Representative quantitative levels of cytokines produced by EBVt-B cells at 24 h after CD150 stimulation with antibody cross-linking, as determined using multiplex cytokine ELISA. (B) Reverse-transcription-quantitative PCR analysis of various inflammatory cytokines at 3, 6, 12 and 24 h after CD150 stimulation with antibody cross-linking in EBVt-B cells. (C) Time course of the quantification of cytokine protein production by EBVt-B cells. (D) Representative quantitative levels of cytokine production in the EBV + Burkitt lymphoma cell lines, IM-9 and Raji at 24 h after CD150 stimulation. * P<0.05, ** P<0.01 vs. mock stimulated with the control UPC-10 Ab. Data are presented as the mean ± SD of three replicates and were analyzed using one-way ANOVA followed by Scheffe's test. All results represent three independent experiments. EBVt-B, Epstein-Barr virus-transformed B (cells); GM-CSF, granulocyte-macrophage colony-stimulating factor.

Techniques Used: Produced, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Virus, Transformation Assay

Production of multiple inflammatory cytokines after treatment with MV or recombinant CD150 protein. The effects of MV#1 and MV#2 on cytokine secretion in (A) EBVt-B cells ( * P<0.05, treated with MV#2 vs. unstimulated EBV-tB cells), (B) Raji cells ( ** P<0.01, treated with MV#1 vs. unstimulated Raji cells) and (C) IM-9 cells ( # P<0.05, treated with MV#2 vs. unstimulated IM-9 cells), as determined using multiplex cytokine ELISA. (D) The effects of rCD150 on cytokine secretion in EBVt-B cells, as determined using multiplex cytokine ELISA. Data are presented as the mean of two independent experiments, and the error bars represent the SD of the mean. ( *** P<0.001, rCD150 vs. unstimulated EBV-tB cells). Comparisons between all individual data were performed using one-way ANOVA followed by Scheffe's test. Ctr, control; EBVt-B, Epstein-Barr virus-transformed B (cells); GM-CSF, granulocyte-macrophage colony-stimulating factor; MV, measles virus; MV#1, MV core protein amino acids 399-525; MV#2, MV core protein amino acids 89-165; rCD150, recombinant CD150.

Figure Legend Snippet: Production of multiple inflammatory cytokines after treatment with MV or recombinant CD150 protein. The effects of MV#1 and MV#2 on cytokine secretion in (A) EBVt-B cells ( * P<0.05, treated with MV#2 vs. unstimulated EBV-tB cells), (B) Raji cells ( ** P<0.01, treated with MV#1 vs. unstimulated Raji cells) and (C) IM-9 cells ( # P<0.05, treated with MV#2 vs. unstimulated IM-9 cells), as determined using multiplex cytokine ELISA. (D) The effects of rCD150 on cytokine secretion in EBVt-B cells, as determined using multiplex cytokine ELISA. Data are presented as the mean of two independent experiments, and the error bars represent the SD of the mean. ( *** P<0.001, rCD150 vs. unstimulated EBV-tB cells). Comparisons between all individual data were performed using one-way ANOVA followed by Scheffe's test. Ctr, control; EBVt-B, Epstein-Barr virus-transformed B (cells); GM-CSF, granulocyte-macrophage colony-stimulating factor; MV, measles virus; MV#1, MV core protein amino acids 399-525; MV#2, MV core protein amino acids 89-165; rCD150, recombinant CD150.

Techniques Used: Recombinant, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Control, Virus, Transformation Assay

Rapid induction of monocyte differentiation into DCs by cytokines secreted from CD150-stimulated EBVt-B cells. (A) Monocytes were incubated with CD150-stimulated EBVt-B cell culture supernatant (total protein concentration, 20 ng/ml) for 7 days and examined for the extent of clustering. Microscopic examination of monocyte clustering and differentiation into DCs over 7 days (original magnification, ×100; scale bars, 100 μ m). (B) Flow cytometry analysis of surface markers expressed on peripheral blood monocytes after a 48 h incubation with culture supernatant derived from CD150-stimulated EBVt-B cells. Culture supernatant derived from EBVt-B cells mock stimulated with an isotype control antibody (UPC-10) was used as a control. The MFI was determined to quantitate the changes in expression levels of cell surface markers on monocytes in a time-dependent manner and is indicated in parentheses within the histogram. (C) Confocal microscopy analysis of monocytes showing changes in the expression of CD14 and CD83, as well as in morphology, after an incubation with CD150-stimulated EBVt-B cell culture supernatant. Scale bars, 10 μ m. (D) Flow cytometry analysis of surface markers on peripheral blood monocytes after a 7-day incubation with culture supernatant derived from MV#2-stimulated EBVt-B cells (total protein concentration, 20 ng/ml). The MFIs (in parentheses) are denoted. Thin curves represent the fluorescence of the isotype control. The results represent four independent experiments. DCs, dendritic cells; DIC, differential interference contrast; EBVt-B, Epstein-Barr virus-transformed B (cells); MFI, mean fluorescence intensity; MV#2, measles virus core protein amino acids 89-165.

Figure Legend Snippet: Rapid induction of monocyte differentiation into DCs by cytokines secreted from CD150-stimulated EBVt-B cells. (A) Monocytes were incubated with CD150-stimulated EBVt-B cell culture supernatant (total protein concentration, 20 ng/ml) for 7 days and examined for the extent of clustering. Microscopic examination of monocyte clustering and differentiation into DCs over 7 days (original magnification, ×100; scale bars, 100 μ m). (B) Flow cytometry analysis of surface markers expressed on peripheral blood monocytes after a 48 h incubation with culture supernatant derived from CD150-stimulated EBVt-B cells. Culture supernatant derived from EBVt-B cells mock stimulated with an isotype control antibody (UPC-10) was used as a control. The MFI was determined to quantitate the changes in expression levels of cell surface markers on monocytes in a time-dependent manner and is indicated in parentheses within the histogram. (C) Confocal microscopy analysis of monocytes showing changes in the expression of CD14 and CD83, as well as in morphology, after an incubation with CD150-stimulated EBVt-B cell culture supernatant. Scale bars, 10 μ m. (D) Flow cytometry analysis of surface markers on peripheral blood monocytes after a 7-day incubation with culture supernatant derived from MV#2-stimulated EBVt-B cells (total protein concentration, 20 ng/ml). The MFIs (in parentheses) are denoted. Thin curves represent the fluorescence of the isotype control. The results represent four independent experiments. DCs, dendritic cells; DIC, differential interference contrast; EBVt-B, Epstein-Barr virus-transformed B (cells); MFI, mean fluorescence intensity; MV#2, measles virus core protein amino acids 89-165.

Techniques Used: Incubation, Cell Culture, Protein Concentration, Flow Cytometry, Derivative Assay, Control, Expressing, Confocal Microscopy, Fluorescence, Virus, Transformation Assay

Neutralization of IL-1α and GM-CSF partially inhibited the differentiation of monocytes into dendritic cells. CD14 + monocytes were incubated with culture supernatant derived from CD150-stimulated or UPC-10 mock-stimulated EBVt-B cells treated with or without neutralizing antibodies against IL-1α or GM-CSF for 3 days. The cells were then harvested, and the expression of various cell surface markers was analyzed using flow cytometry. The MFIs (in parentheses) are denoted. Thin curves represent the fluorescence of the isotype control. The results represent three independent experiments. EBVt-B, Epstein-Barr virus-transformed B (cells); GM-CSF, granulocyte-macrophage colony-stimulating factor.

Figure Legend Snippet: Neutralization of IL-1α and GM-CSF partially inhibited the differentiation of monocytes into dendritic cells. CD14 + monocytes were incubated with culture supernatant derived from CD150-stimulated or UPC-10 mock-stimulated EBVt-B cells treated with or without neutralizing antibodies against IL-1α or GM-CSF for 3 days. The cells were then harvested, and the expression of various cell surface markers was analyzed using flow cytometry. The MFIs (in parentheses) are denoted. Thin curves represent the fluorescence of the isotype control. The results represent three independent experiments. EBVt-B, Epstein-Barr virus-transformed B (cells); GM-CSF, granulocyte-macrophage colony-stimulating factor.

Techniques Used: Neutralization, Incubation, Derivative Assay, Expressing, Flow Cytometry, Fluorescence, Control, Virus, Transformation Assay

Effects of CD150 knockdown on inflammatory cytokine mRNA and protein levels. After 24 h of transfection with the CD150 siRNA or control siRNA, the cells were incubated with an anti-CD150 monoclonal antibody or isotype control, followed by a cross-linking secondary antibody. After 24 h, the cells and supernatant were collected for further analysis. (A) Reverse transcription-quantitative PCR analysis showing reduced cytokine mRNA levels following CD150 knockdown. Data are presented as the mean ± SD of triplicate samples. (B) Multiplex cytokine ELISA results confirming decreased cytokine secretion post-CD150 knockdown. Data are presented as the mean ± SD of duplicate samples. * P<0.05, si-ctr./anti-CD150 vs. si-CD150/anti-CD150. Comparisons between all individual data were performed using one-way ANOVA followed by Scheffe's test. The results represent three independent experiments. ctr, control; GM-CSF, granulocyte-macrophage colony-stimulating factor; siRNA, small interfering RNA.

Figure Legend Snippet: Effects of CD150 knockdown on inflammatory cytokine mRNA and protein levels. After 24 h of transfection with the CD150 siRNA or control siRNA, the cells were incubated with an anti-CD150 monoclonal antibody or isotype control, followed by a cross-linking secondary antibody. After 24 h, the cells and supernatant were collected for further analysis. (A) Reverse transcription-quantitative PCR analysis showing reduced cytokine mRNA levels following CD150 knockdown. Data are presented as the mean ± SD of triplicate samples. (B) Multiplex cytokine ELISA results confirming decreased cytokine secretion post-CD150 knockdown. Data are presented as the mean ± SD of duplicate samples. * P<0.05, si-ctr./anti-CD150 vs. si-CD150/anti-CD150. Comparisons between all individual data were performed using one-way ANOVA followed by Scheffe's test. The results represent three independent experiments. ctr, control; GM-CSF, granulocyte-macrophage colony-stimulating factor; siRNA, small interfering RNA.

Techniques Used: Knockdown, Transfection, Control, Incubation, Reverse Transcription, Real-time Polymerase Chain Reaction, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Small Interfering RNA

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Journal: International Journal of Molecular Medicine

Article Title: CD150-dependent activation of EBV-transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines

doi: 10.3892/ijmm.2024.5403

Figure Lengend Snippet: Sequences of the oligonucleotide primers used for RT-PCR and RT-qPCR.

Article Snippet: The three experimentally verified CD150 siRNA sequences were selected from the Bioneer siRNA database, and the target sequences are listed in .

Techniques:

Journal: International Journal of Molecular Medicine

Article Title: CD150-dependent activation of EBV-transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines

doi: 10.3892/ijmm.2024.5403

Figure Lengend Snippet: siRNAs used in transfection.

Article Snippet: The three experimentally verified CD150 siRNA sequences were selected from the Bioneer siRNA database, and the target sequences are listed in .

Techniques: Transfection, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: CD150-dependent activation of EBV-transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines

doi: 10.3892/ijmm.2024.5403

Figure Lengend Snippet: Dynamics of CD150 expression in EBVt-B cells. Resting B cells purified from human peripheral blood were immortalized with medium containing EBV particles. (A) Weekly flow cytometry analysis of EBVt-B cells stained with PE-conjugated anti-human CD150 and FITC-conjugated anti-human CD20 antibodies. The left panels show surface expression and the right panels show intracellular expression. (B) Confocal microscopy analysis showing the upregulation of CD150 expression and colocalization of CD150 with CD20 on the cell surface and in the cytoplasm of EBVt-B cells. Scale bars, 10 μ m. (C) Reverse transcription-quantitative PCR results showing CD150 mRNA levels relative to β-actin at 4 weeks post infection. * P<0.05, ** P<0.01 vs. naïve B cells. Data are presented as the mean ± SD of three replicates and were analyzed using one-way ANOVA followed by Scheffe's test. The results represent three independent experiments. EBV, Epstein-Barr virus; EBVt-B, EBV-transformed B (cells); PBMCs, peripheral blood mononuclear cells; wk, week.

Article Snippet: The three experimentally verified CD150 siRNA sequences were selected from the Bioneer siRNA database, and the target sequences are listed in .

Techniques: Expressing, Purification, Flow Cytometry, Staining, Confocal Microscopy, Reverse Transcription, Real-time Polymerase Chain Reaction, Infection, Virus, Transformation Assay

Journal: International Journal of Molecular Medicine

Article Title: CD150-dependent activation of EBV-transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines

doi: 10.3892/ijmm.2024.5403

Figure Lengend Snippet: Effects of CD150 stimulation on inflammatory cytokine secretion. (A) Representative quantitative levels of cytokines produced by EBVt-B cells at 24 h after CD150 stimulation with antibody cross-linking, as determined using multiplex cytokine ELISA. (B) Reverse-transcription-quantitative PCR analysis of various inflammatory cytokines at 3, 6, 12 and 24 h after CD150 stimulation with antibody cross-linking in EBVt-B cells. (C) Time course of the quantification of cytokine protein production by EBVt-B cells. (D) Representative quantitative levels of cytokine production in the EBV + Burkitt lymphoma cell lines, IM-9 and Raji at 24 h after CD150 stimulation. * P<0.05, ** P<0.01 vs. mock stimulated with the control UPC-10 Ab. Data are presented as the mean ± SD of three replicates and were analyzed using one-way ANOVA followed by Scheffe's test. All results represent three independent experiments. EBVt-B, Epstein-Barr virus-transformed B (cells); GM-CSF, granulocyte-macrophage colony-stimulating factor.

Article Snippet: The three experimentally verified CD150 siRNA sequences were selected from the Bioneer siRNA database, and the target sequences are listed in .

Techniques: Produced, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Virus, Transformation Assay

Journal: International Journal of Molecular Medicine

Article Title: CD150-dependent activation of EBV-transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines

doi: 10.3892/ijmm.2024.5403

Figure Lengend Snippet: Production of multiple inflammatory cytokines after treatment with MV or recombinant CD150 protein. The effects of MV#1 and MV#2 on cytokine secretion in (A) EBVt-B cells ( * P<0.05, treated with MV#2 vs. unstimulated EBV-tB cells), (B) Raji cells ( ** P<0.01, treated with MV#1 vs. unstimulated Raji cells) and (C) IM-9 cells ( # P<0.05, treated with MV#2 vs. unstimulated IM-9 cells), as determined using multiplex cytokine ELISA. (D) The effects of rCD150 on cytokine secretion in EBVt-B cells, as determined using multiplex cytokine ELISA. Data are presented as the mean of two independent experiments, and the error bars represent the SD of the mean. ( *** P<0.001, rCD150 vs. unstimulated EBV-tB cells). Comparisons between all individual data were performed using one-way ANOVA followed by Scheffe's test. Ctr, control; EBVt-B, Epstein-Barr virus-transformed B (cells); GM-CSF, granulocyte-macrophage colony-stimulating factor; MV, measles virus; MV#1, MV core protein amino acids 399-525; MV#2, MV core protein amino acids 89-165; rCD150, recombinant CD150.

Article Snippet: The three experimentally verified CD150 siRNA sequences were selected from the Bioneer siRNA database, and the target sequences are listed in .

Techniques: Recombinant, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Control, Virus, Transformation Assay

Journal: International Journal of Molecular Medicine

Article Title: CD150-dependent activation of EBV-transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines

doi: 10.3892/ijmm.2024.5403

Figure Lengend Snippet: Rapid induction of monocyte differentiation into DCs by cytokines secreted from CD150-stimulated EBVt-B cells. (A) Monocytes were incubated with CD150-stimulated EBVt-B cell culture supernatant (total protein concentration, 20 ng/ml) for 7 days and examined for the extent of clustering. Microscopic examination of monocyte clustering and differentiation into DCs over 7 days (original magnification, ×100; scale bars, 100 μ m). (B) Flow cytometry analysis of surface markers expressed on peripheral blood monocytes after a 48 h incubation with culture supernatant derived from CD150-stimulated EBVt-B cells. Culture supernatant derived from EBVt-B cells mock stimulated with an isotype control antibody (UPC-10) was used as a control. The MFI was determined to quantitate the changes in expression levels of cell surface markers on monocytes in a time-dependent manner and is indicated in parentheses within the histogram. (C) Confocal microscopy analysis of monocytes showing changes in the expression of CD14 and CD83, as well as in morphology, after an incubation with CD150-stimulated EBVt-B cell culture supernatant. Scale bars, 10 μ m. (D) Flow cytometry analysis of surface markers on peripheral blood monocytes after a 7-day incubation with culture supernatant derived from MV#2-stimulated EBVt-B cells (total protein concentration, 20 ng/ml). The MFIs (in parentheses) are denoted. Thin curves represent the fluorescence of the isotype control. The results represent four independent experiments. DCs, dendritic cells; DIC, differential interference contrast; EBVt-B, Epstein-Barr virus-transformed B (cells); MFI, mean fluorescence intensity; MV#2, measles virus core protein amino acids 89-165.

Article Snippet: The three experimentally verified CD150 siRNA sequences were selected from the Bioneer siRNA database, and the target sequences are listed in .

Techniques: Incubation, Cell Culture, Protein Concentration, Flow Cytometry, Derivative Assay, Control, Expressing, Confocal Microscopy, Fluorescence, Virus, Transformation Assay

Journal: International Journal of Molecular Medicine

Article Title: CD150-dependent activation of EBV-transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines

doi: 10.3892/ijmm.2024.5403

Figure Lengend Snippet: Neutralization of IL-1α and GM-CSF partially inhibited the differentiation of monocytes into dendritic cells. CD14 + monocytes were incubated with culture supernatant derived from CD150-stimulated or UPC-10 mock-stimulated EBVt-B cells treated with or without neutralizing antibodies against IL-1α or GM-CSF for 3 days. The cells were then harvested, and the expression of various cell surface markers was analyzed using flow cytometry. The MFIs (in parentheses) are denoted. Thin curves represent the fluorescence of the isotype control. The results represent three independent experiments. EBVt-B, Epstein-Barr virus-transformed B (cells); GM-CSF, granulocyte-macrophage colony-stimulating factor.

Article Snippet: The three experimentally verified CD150 siRNA sequences were selected from the Bioneer siRNA database, and the target sequences are listed in .

Techniques: Neutralization, Incubation, Derivative Assay, Expressing, Flow Cytometry, Fluorescence, Control, Virus, Transformation Assay

Journal: International Journal of Molecular Medicine

Article Title: CD150-dependent activation of EBV-transformed B cells induces the differentiation of peripheral blood monocytes via the secretion of multiple cytokines

doi: 10.3892/ijmm.2024.5403

Figure Lengend Snippet: Effects of CD150 knockdown on inflammatory cytokine mRNA and protein levels. After 24 h of transfection with the CD150 siRNA or control siRNA, the cells were incubated with an anti-CD150 monoclonal antibody or isotype control, followed by a cross-linking secondary antibody. After 24 h, the cells and supernatant were collected for further analysis. (A) Reverse transcription-quantitative PCR analysis showing reduced cytokine mRNA levels following CD150 knockdown. Data are presented as the mean ± SD of triplicate samples. (B) Multiplex cytokine ELISA results confirming decreased cytokine secretion post-CD150 knockdown. Data are presented as the mean ± SD of duplicate samples. * P<0.05, si-ctr./anti-CD150 vs. si-CD150/anti-CD150. Comparisons between all individual data were performed using one-way ANOVA followed by Scheffe's test. The results represent three independent experiments. ctr, control; GM-CSF, granulocyte-macrophage colony-stimulating factor; siRNA, small interfering RNA.

Article Snippet: The three experimentally verified CD150 siRNA sequences were selected from the Bioneer siRNA database, and the target sequences are listed in .

Techniques: Knockdown, Transfection, Control, Incubation, Reverse Transcription, Real-time Polymerase Chain Reaction, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Small Interfering RNA

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